Laccaria bicolor MiSSP8 is a small-secreted protein decisive for the establishment of the ectomycorrhizal symbiosis.
Identifieur interne : 000859 ( Main/Exploration ); précédent : 000858; suivant : 000860Laccaria bicolor MiSSP8 is a small-secreted protein decisive for the establishment of the ectomycorrhizal symbiosis.
Auteurs : Clément Pellegrin [France] ; Yohann Daguerre [France] ; Joske Ruytinx [France] ; Frédéric Guinet [France] ; Minna Kemppainen [Argentine] ; Nicolas Frei Dit Frey [France] ; Virginie Puech-Pagès [France] ; Arnaud Hecker [France] ; Alejandro G. Pardo [Argentine] ; Francis M. Martin [France] ; Claire Veneault-Fourrey [France]Source :
- Environmental microbiology [ 1462-2920 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Populus, Racines de plante.
- métabolisme : Hyphae, Protéines fongiques.
- physiologie : Laccaria, Mycorhizes, Protéines fongiques.
- Symbiose, Écosystème.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Fungal Proteins.
- chemical , physiology : Fungal Proteins.
- metabolism : Hyphae.
- microbiology : Plant Roots, Populus.
- physiology : Laccaria, Mycorrhizae.
- Ecosystem, Symbiosis.
Abstract
The ectomycorrhizal symbiosis is a predominant tree-microbe interaction in forest ecosystems sustaining tree growth and health. Its establishment and functioning implies a long-term and intimate relationship between the soil-borne fungi and the roots of trees. Mycorrhiza-induced Small-Secreted Proteins (MiSSPs) are hypothesized as keystone symbiotic proteins, required to set up the symbiosis by modifying the host metabolism and/or building the symbiotic interfaces. L. bicolor MiSSP8 is the third most highly induced MiSSPs in symbiotic tissues and it is also expressed in fruiting bodies. The MiSSP8-RNAi knockdown mutants are strongly impaired in their mycorrhization ability with Populus, with the lack of fungal mantle and Hartig net development due to the lack of hyphal aggregation. MiSSP8 C-terminus displays a repetitive motif containing a kexin cleavage site, recognized by KEX2 in vitro. This suggests MiSSP8 protein might be cleaved into small peptides. Moreover, the MiSSP8 repetitive motif is found in other proteins predicted secreted by both saprotrophic and ectomycorrhizal fungi. Thus, our data indicate that MiSSP8 is a small-secreted protein involved at early stages of ectomycorrhizal symbiosis, likely by regulating hyphal aggregation and pseudoparenchyma formation.
DOI: 10.1111/1462-2920.14727
PubMed: 31260142
Affiliations:
- Argentine, France
- Grand Est, Lorraine (région), Midi-Pyrénées, Occitanie (région administrative)
- Castanet Tolosan, Champenoux, Metz, Nancy
- Université de Lorraine
Links toward previous steps (curation, corpus...)
Le document en format XML
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France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet Tolosan</settlement></placeName></affiliation></author><author><name sortKey="Puech Pages, Virginie" sort="Puech Pages, Virginie" uniqKey="Puech Pages V" first="Virginie" last="Puech-Pagès">Virginie Puech-Pagès</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, 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Pardo</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnologıa, Universidad Nacional de Quilmes and CONICET, Roque Sáenz Peña 352, B1876, Bernal, Provincia de Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnologıa, Universidad Nacional de Quilmes and CONICET, Roque Sáenz Peña 352, B1876, Bernal, Provincia de Buenos Aires</wicri:regionArea><wicri:noRegion>Provincia de Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Martin, Francis M" sort="Martin, Francis M" uniqKey="Martin F" first="Francis M" last="Martin">Francis M. Martin</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy</wicri:regionArea><wicri:noRegion>lès Nancy</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author><author><name sortKey="Veneault Fourrey, Claire" sort="Veneault Fourrey, Claire" uniqKey="Veneault Fourrey C" first="Claire" last="Veneault-Fourrey">Claire Veneault-Fourrey</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy</wicri:regionArea><wicri:noRegion>lès Nancy</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31260142</idno><idno type="pmid">31260142</idno><idno type="doi">10.1111/1462-2920.14727</idno><idno type="wicri:Area/Main/Corpus">000816</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000816</idno><idno type="wicri:Area/Main/Curation">000816</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000816</idno><idno type="wicri:Area/Main/Exploration">000816</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Laccaria bicolor MiSSP8 is a small-secreted protein decisive for the establishment of the ectomycorrhizal symbiosis.</title><author><name sortKey="Pellegrin, Clement" sort="Pellegrin, Clement" uniqKey="Pellegrin C" first="Clément" last="Pellegrin">Clément Pellegrin</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy</wicri:regionArea><wicri:noRegion>lès Nancy</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author><author><name sortKey="Daguerre, Yohann" sort="Daguerre, Yohann" uniqKey="Daguerre Y" first="Yohann" last="Daguerre">Yohann Daguerre</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy</wicri:regionArea><wicri:noRegion>lès Nancy</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author><author><name sortKey="Ruytinx, Joske" sort="Ruytinx, Joske" uniqKey="Ruytinx J" first="Joske" last="Ruytinx">Joske Ruytinx</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy</wicri:regionArea><wicri:noRegion>lès Nancy</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author><author><name sortKey="Guinet, Frederic" sort="Guinet, Frederic" uniqKey="Guinet F" first="Frédéric" last="Guinet">Frédéric Guinet</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy</wicri:regionArea><wicri:noRegion>lès Nancy</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author><author><name sortKey="Kemppainen, Minna" sort="Kemppainen, Minna" uniqKey="Kemppainen M" first="Minna" last="Kemppainen">Minna Kemppainen</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnologıa, Universidad Nacional de Quilmes and CONICET, Roque Sáenz Peña 352, B1876, Bernal, Provincia de Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnologıa, Universidad Nacional de Quilmes and CONICET, Roque Sáenz Peña 352, B1876, Bernal, Provincia de Buenos Aires</wicri:regionArea><wicri:noRegion>Provincia de Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Frey, Nicolas Frei Dit" sort="Frey, Nicolas Frei Dit" uniqKey="Frey N" first="Nicolas Frei Dit" last="Frey">Nicolas Frei Dit Frey</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet Tolosan</settlement></placeName></affiliation></author><author><name sortKey="Puech Pages, Virginie" sort="Puech Pages, Virginie" uniqKey="Puech Pages V" first="Virginie" last="Puech-Pagès">Virginie Puech-Pagès</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet Tolosan</settlement></placeName></affiliation></author><author><name sortKey="Hecker, Arnaud" sort="Hecker, Arnaud" uniqKey="Hecker A" first="Arnaud" last="Hecker">Arnaud Hecker</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy</wicri:regionArea><wicri:noRegion>lès Nancy</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author><author><name sortKey="Pardo, Alejandro G" sort="Pardo, Alejandro G" uniqKey="Pardo A" first="Alejandro G" last="Pardo">Alejandro G. Pardo</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnologıa, Universidad Nacional de Quilmes and CONICET, Roque Sáenz Peña 352, B1876, Bernal, Provincia de Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnologıa, Universidad Nacional de Quilmes and CONICET, Roque Sáenz Peña 352, B1876, Bernal, Provincia de Buenos Aires</wicri:regionArea><wicri:noRegion>Provincia de Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Martin, Francis M" sort="Martin, Francis M" uniqKey="Martin F" first="Francis M" last="Martin">Francis M. Martin</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy</wicri:regionArea><wicri:noRegion>lès Nancy</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author><author><name sortKey="Veneault Fourrey, Claire" sort="Veneault Fourrey, Claire" uniqKey="Veneault Fourrey C" first="Claire" last="Veneault-Fourrey">Claire Veneault-Fourrey</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy</wicri:regionArea><wicri:noRegion>lès Nancy</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author></analytic><series><title level="j">Environmental microbiology</title><idno type="eISSN">1462-2920</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ecosystem (MeSH)</term><term>Fungal Proteins (metabolism)</term><term>Fungal Proteins (physiology)</term><term>Hyphae (metabolism)</term><term>Laccaria (physiology)</term><term>Mycorrhizae (physiology)</term><term>Plant Roots (microbiology)</term><term>Populus (microbiology)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Hyphae (métabolisme)</term><term>Laccaria (physiologie)</term><term>Mycorhizes (physiologie)</term><term>Populus (microbiologie)</term><term>Protéines fongiques (métabolisme)</term><term>Protéines fongiques (physiologie)</term><term>Racines de plante (microbiologie)</term><term>Symbiose (MeSH)</term><term>Écosystème (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hyphae</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Populus</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Roots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Hyphae</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Laccaria</term><term>Mycorhizes</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Laccaria</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Ecosystem</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Symbiose</term><term>Écosystème</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ectomycorrhizal symbiosis is a predominant tree-microbe interaction in forest ecosystems sustaining tree growth and health. Its establishment and functioning implies a long-term and intimate relationship between the soil-borne fungi and the roots of trees. Mycorrhiza-induced Small-Secreted Proteins (MiSSPs) are hypothesized as keystone symbiotic proteins, required to set up the symbiosis by modifying the host metabolism and/or building the symbiotic interfaces. L. bicolor MiSSP8 is the third most highly induced MiSSPs in symbiotic tissues and it is also expressed in fruiting bodies. The MiSSP8-RNAi knockdown mutants are strongly impaired in their mycorrhization ability with Populus, with the lack of fungal mantle and Hartig net development due to the lack of hyphal aggregation. MiSSP8 C-terminus displays a repetitive motif containing a kexin cleavage site, recognized by KEX2 in vitro. This suggests MiSSP8 protein might be cleaved into small peptides. Moreover, the MiSSP8 repetitive motif is found in other proteins predicted secreted by both saprotrophic and ectomycorrhizal fungi. Thus, our data indicate that MiSSP8 is a small-secreted protein involved at early stages of ectomycorrhizal symbiosis, likely by regulating hyphal aggregation and pseudoparenchyma formation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31260142</PMID><DateCompleted><Year>2020</Year><Month>04</Month><Day>29</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>29</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1462-2920</ISSN><JournalIssue CitedMedium="Internet"><Volume>21</Volume><Issue>10</Issue><PubDate><Year>2019</Year><Month>10</Month></PubDate></JournalIssue><Title>Environmental microbiology</Title><ISOAbbreviation>Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Laccaria bicolor MiSSP8 is a small-secreted protein decisive for the establishment of the ectomycorrhizal symbiosis.</ArticleTitle><Pagination><MedlinePgn>3765-3779</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/1462-2920.14727</ELocationID><Abstract><AbstractText>The ectomycorrhizal symbiosis is a predominant tree-microbe interaction in forest ecosystems sustaining tree growth and health. Its establishment and functioning implies a long-term and intimate relationship between the soil-borne fungi and the roots of trees. Mycorrhiza-induced Small-Secreted Proteins (MiSSPs) are hypothesized as keystone symbiotic proteins, required to set up the symbiosis by modifying the host metabolism and/or building the symbiotic interfaces. L. bicolor MiSSP8 is the third most highly induced MiSSPs in symbiotic tissues and it is also expressed in fruiting bodies. The MiSSP8-RNAi knockdown mutants are strongly impaired in their mycorrhization ability with Populus, with the lack of fungal mantle and Hartig net development due to the lack of hyphal aggregation. MiSSP8 C-terminus displays a repetitive motif containing a kexin cleavage site, recognized by KEX2 in vitro. This suggests MiSSP8 protein might be cleaved into small peptides. Moreover, the MiSSP8 repetitive motif is found in other proteins predicted secreted by both saprotrophic and ectomycorrhizal fungi. Thus, our data indicate that MiSSP8 is a small-secreted protein involved at early stages of ectomycorrhizal symbiosis, likely by regulating hyphal aggregation and pseudoparenchyma formation.</AbstractText><CopyrightInformation>© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pellegrin</LastName><ForeName>Clément</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Daguerre</LastName><ForeName>Yohann</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ruytinx</LastName><ForeName>Joske</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guinet</LastName><ForeName>Frédéric</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kemppainen</LastName><ForeName>Minna</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnologıa, Universidad Nacional de Quilmes and CONICET, Roque Sáenz Peña 352, B1876, Bernal, Provincia de Buenos Aires, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Frey</LastName><ForeName>Nicolas Frei Dit</ForeName><Initials>NFD</Initials><AffiliationInfo><Affiliation>Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Puech-Pagès</LastName><ForeName>Virginie</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hecker</LastName><ForeName>Arnaud</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pardo</LastName><ForeName>Alejandro G</ForeName><Initials>AG</Initials><AffiliationInfo><Affiliation>Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnologıa, Universidad Nacional de Quilmes and CONICET, Roque Sáenz Peña 352, B1876, Bernal, Provincia de Buenos Aires, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martin</LastName><ForeName>Francis M</ForeName><Initials>FM</Initials><AffiliationInfo><Affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Veneault-Fourrey</LastName><ForeName>Claire</ForeName><Initials>C</Initials><Identifier Source="ORCID">0000-0001-9778-2659</Identifier><AffiliationInfo><Affiliation>INRA, UMR1136, Interactions Arbres/microorganismes, Centre Grand-Est, Champenoux, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR 1136, Interactions Arbres/Microorganismes (IAM), Faculté des Sciences et Technologies, Université de Lorraine, Vandœuvre, lès Nancy, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>European Fund for Regional Development</Agency><Country>International</Country></Grant><Grant><GrantID>Plant-Microbe Interfaces Scientific Focus Area</GrantID><Agency>Genomic Science Program, US Department of Energy, Office of Science, Biological and Environmental Research</Agency><Country>International</Country></Grant><Grant><GrantID>ANR-11-LABX-0002_ARBRE</GrantID><Agency>Laboratory of Excellence ARBRE</Agency><Country>International</Country></Grant><Grant><Agency>Région Lorraine Research council</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>07</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Environ Microbiol</MedlineTA><NlmUniqueID>100883692</NlmUniqueID><ISSNLinking>1462-2912</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017753" MajorTopicYN="N">Ecosystem</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055399" MajorTopicYN="N">Laccaria</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>05</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>06</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>06</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31260142</ArticleId><ArticleId IdType="doi">10.1111/1462-2920.14727</ArticleId></ArticleIdList><ReferenceList><Title>References</Title><Reference><Citation>Akum, F.N., Steinbrenner, J., Biedenkopf, D., Imani, J., and Kogel, K.-H. (2015) The Piriformospora indica effector PIIN_08944 promotes the mutualistic Sebacinalean symbiosis. Front Plant Sci 6: 906.</Citation></Reference><Reference><Citation>Balestrini, R., and Kottke, I. (2016) Structure and development of ectomycorrhizal roots. In Molecular Mycorrhizal Symbiosis. Oxford: Wiley-Blackwell, pp. 47-61.</Citation></Reference><Reference><Citation>Berg, G., Mahnert, A., and Moissl-Eichinger, C. (2014) Beneficial effects of plant-associated microbes on indoor microbiomes and human health? Front Microbiol 5: 491. https://doi.org/10.3389/fmicb.2014.00491.</Citation></Reference><Reference><Citation>Berg, S., Pimenov, A., Palmer, C., Emmerson, M., and Jonsson, T. (2015) Ecological communities are vulnerable to realistic extinction sequences. Oikos 124: 486-496.</Citation></Reference><Reference><Citation>Brundrett, M.C. (2009) Mycorrhizal associations and other means of nutrition of vascular plants: understanding the global diversity of host plants by resolving conflicting information and developing reliable means of diagnosis. Plant and Soil 320: 37-77.</Citation></Reference><Reference><Citation>Brunner, I., and Scheidegger, C. (1992) Ontogeny of synthesized Picea abies (L.) Karst.-Hebeloma crustuliniforme (Bull. ex St Amans) Quél. ectomycorrhizas. New Phytol 120: 359-369.</Citation></Reference><Reference><Citation>Buée, M., Reich, M., Murat, C., Morin, E., Nilsson, R.H., Uroz, S., and Martin, F. (2009) 454 pyrosequencing analyses of forest soils reveal an unexpectedly high fungal diversity. New Phytol 184: 449-456.</Citation></Reference><Reference><Citation>de Castro, E., Sigrist, C.J.A., Gattiker, A., Bulliard, V., Langendijk-Genevaux, P.S., Gasteiger, E., et al. (2006) ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins. Nucleic Acids Res 34: W362-W365.</Citation></Reference><Reference><Citation>Daguerre, Y., Plett, J.M., and Veneault-Fourrey, C. (2017) Signaling pathways driving the development of ectomycorrhizal symbiosis. In Molecular Mycorrhizal Symbiosis, Martin, F. (ed). Hoboken, NJ: John Wiley & Sons, Inc, pp. 141-157.</Citation></Reference><Reference><Citation>van Der Heijden, M.G.A., Klironomos, J.N., and Ursic, M. (1998) Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396: 69-72.</Citation></Reference><Reference><Citation>Dexheimer, J., Gerard, J., and Genet, P. (1994) Study of transformations of the root system of Eucalyptus globulus associated with Pisolithus tinctorius. I. Aptitude to mycorrhization of different kinds of roots. Phytomorphology 44: 235-245.</Citation></Reference><Reference><Citation>Di Battista, C., Selosse, M.-A., Bouchard, D., Stenström, E., and Le Tacon, F. (1996) Variations in symbiotic efficiency, phenotypic characters and ploidylevel among different isolates of the ectomycorrhizal basidiomycete Laccaria bicolor strain S 238. Mycological Research 100: 1315-1324.</Citation></Reference><Reference><Citation>Ditengou, F.A., Müller, A., Rosenkranz, M., Felten, J., Lasok, H., van Doorn, M.M., et al. (2015) Volatile signalling by sesquiterpenes from ectomycorrhizal fungi reprogrammes root architecture. Nat Commun 6: 6279.</Citation></Reference><Reference><Citation>Ding, W., Liu, W.-Q., Jia, Y., Li, Y., van der Donk, W.A., and Zhang, Q. (2016) Biosynthetic investigation of phomopsins reveals a widespread pathway for ribosomal natural products in Ascomycetes. Proc Natl Acad Sci 113: 3521-3526.</Citation></Reference><Reference><Citation>Doré, J., Perraud, M., Dieryckx, C., Kohler, A., Morin, E., Henrissat, B., et al. (2015) Comparative genomics, proteomics and transcriptomics give new insight into the exoproteome of the basidiomycete Hebeloma cylindrosporum and its involvement in ectomycorrhizal symbiosis. New Phytol 208: 1169-1187.</Citation></Reference><Reference><Citation>Drozdetskiy, A., Cole, C., Procter, J., and Barton, G.J. (2015) JPred4: a protein secondary structure prediction server. Nucleic Acids Res 43: 389-394.</Citation></Reference><Reference><Citation>Felten, J., Kohler, A., Morin, E., Bhalerao, R.P., Palme, K., Martin, F., et al. (2009) The ectomycorrhizal fungus Laccaria bicolor stimulates lateral root formation in poplar and Arabidopsis through auxin transport and signaling. Plant Physiol 151: 1991-2005.</Citation></Reference><Reference><Citation>Fierer, N., Breitbart, M., Nulton, J., Salamon, P., Lozupone, C., Jones, R., et al. (2007) Metagenomic and small-subunit rRNA analyses reveal the genetic diversity of bacteria, archaea, fungi, and viruses in soil. Appl Environ Microbiol 73: 7059-7066.</Citation></Reference><Reference><Citation>Frith, M.C., Saunders, N.F.W., Kobe, B., and Bailey, T.L. (2008) Discovering sequence motifs with arbitrary insertions and deletions. PLoS Comput Biol 4: e1000071.</Citation></Reference><Reference><Citation>Genre, A., and Bonfante, P. (2012) The interface between plants and mycorrhizal fungi: nutrient exchange, signaling and cell organization. In The Mycota, IX: Fungal Associations, 2nd ed, Hock, B. (ed). Berlin, Heidelberg: Springer Berlin Heidelberg, pp. 39-49.</Citation></Reference><Reference><Citation>Gietz, R.D., and Schiestl, R.H. (2008) High-efficiency yeast transformation using the LiAc / SS carrier DNA/PEG method. Nat Protoc 2: 31-35.</Citation></Reference><Reference><Citation>Hess, J., Skrede, I., Chaib De Mares, M., Hainaut, M., Henrissat, B., and Pringle, A. (2018) Rapid divergence of genome architectures following the origin of an ectomycorrhizal symbiosis in the genus amanita. Mol Biol Evol 35: 2786-2804.https://doi.org/10.1093/molbev/msy179.</Citation></Reference><Reference><Citation>Homer, C.M., Summers, D.K., Goranov, A.I., Clarke, S.C., Wiesner, D.L., Diedrich, J.K., et al. (2016) Intracellular action of a secreted peptide required for fungal virulence. Cell Host Microbe 19: 849-864.</Citation></Reference><Reference><Citation>Jacobs, K.A., Collins-Racie, L.A., Colbert, M., Duckett, M., Golden-Fleet, M., Kelleher, K., et al. (1997) A genetic selection for isolating cDNAs encoding secreted proteins. Gene 198: 289-296.</Citation></Reference><Reference><Citation>Kemppainen, M.J., and Pardo, A.G. (2010) pHg/pSILBAγ vector system for efficient gene silencing in homobasidiomycetes: optimization of ihpRNA - triggering in the mycorrhizal fungus Laccaria bicolor. J Microbial Biotechnol 3: 178-200.</Citation></Reference><Reference><Citation>Kemppainen, M., Circosta, A., Tagu, D., Martin, F., and Pardo, A.G. (2005) Agrobacterium-mediated transformation of the ectomycorrhizal symbiont Laccaria bicolor S238N. Mycorrhiza 16: 19-22.</Citation></Reference><Reference><Citation>Kemppainen, M., Duplessis, S., Martin, F., and Pardo, A.G. (2008) T-DNA insertion, plasmid rescue and integration analysis in the model mycorrhizal fungus Laccaria bicolor. J Microbial Biotechnol 1: 258-269.</Citation></Reference><Reference><Citation>Kemppainen, M., Duplessis, S., Martin, F., and Pardo, A.G. (2009) RNA silencing in the model mycorrhizal fungus Laccaria bicolor: gene knock-down of nitrate reductase results in inhibition of symbiosis with Populus. Environ Microbiol 11: 1878-1896.</Citation></Reference><Reference><Citation>Kloppholz, S., Kuhn, H., and Requena, N. (2011) A secreted fungal effector of Glomus intraradices promotes symbiotic biotrophy. Curr Biol 21: 1204-1209.</Citation></Reference><Reference><Citation>Kohler, A., Kuo, A., Nagy, L.G., Morin, E., Barry, K.W., Buscot, F., et al. (2015) Convergent losses of decay mechanisms and rapid turnover of symbiosis genes in mycorrhizal mutualists. Nat Genet 47: 410-415.</Citation></Reference><Reference><Citation>Krause, K., Henke, C., Asiimwe, T., Ulbricht, A., Klemmer, S., Schachtschabel, D., et al. (2015) Biosynthesis and secretion of indole-3-acetic acid and its morphological effects on Tricholoma vaccinum-spruce ectomycorrhiza. Appl Environ Microbiol 81: 7003-7011.</Citation></Reference><Reference><Citation>Kües, U., and Navarro-González, M. (2015) How do Agaricomycetes shape their fruiting bodies? 1. Morphological aspects of development. Fungal Biol Rev 29: 63-97.</Citation></Reference><Reference><Citation>Kyte, J., and Doolittle, R.F. (1992) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105-132.</Citation></Reference><Reference><Citation>Lakkireddy, K., Navarro-González, M., Velagapudi, R., and Kües, U. (2011) Proteins expressed during hyphal aggregation for fruiting body formation in basidiomycetes. In Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products (ICMBMP7) 1: P82-P94.</Citation></Reference><Reference><Citation>Le Marquer, M., San Clemente, H., Roux, C., Savelli, B., and Frei dit Frey, N. (2019) Identification of new signalling peptides through a genome-wide survey of 250 fungal secretomes. BMC Genomics 20: 64. https://doi.org/10.1186/s12864-018-5414-2.</Citation></Reference><Reference><Citation>Lindahl, B.D., and Tunlid, A. (2015) Ectomycorrhizal fungi - potential organic matter decomposers, yet not saprotrophs. New Phytol 205: 1443-1447.</Citation></Reference><Reference><Citation>Liu, Y., Guang, G., Mitsukawa, N., Oosumi, T., and Whittier, R.F. (1995) Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 8: 457-463.</Citation></Reference><Reference><Citation>Lo Presti, L., Lanver, D., Schweizer, G., Tanaka, S., Liang, L., Tollot, M., et al. (2015) Fungal effectors and plant susceptibility. Annu Rev Plant Biol 66: 513-545.</Citation></Reference><Reference><Citation>Martin, F., Laurent, P., de Carvalho, D., Voiblet, C., Balestrini, R., Bonfante, P., and Tagu, D. (1999) Cell wall proteins of the ectomycorrhizal basidiomycete Pisolithus tinctorius: identification, function, and expression in symbiosis. Fungal Genet Biol 27: 161-174.</Citation></Reference><Reference><Citation>Martin, F., Aerts, A., Ahrén, D., Brun, A., Danchin, E.G.J., Duchaussoy, F., et al. (2008) The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis. Nature 452: 88-92.</Citation></Reference><Reference><Citation>Martin, F., Kohler, A., Murat, C., Veneault-Fourrey, C., and Hibbett, D.S. (2016) Unearthing the roots of ectomycorrhizal symbioses. Nat Rev Microbiol 14: 760-773.</Citation></Reference><Reference><Citation>Massicotte, H.B., Peterson, R.L., Ackerley, C.A., and Melville, L.H. (1990) Structure and ontogeny of Betula alleghaniensis - Pisolithus tinctorius ectomycorrhizae. Can J Bot 68: 579-593.</Citation></Reference><Reference><Citation>Mizuno, K., Nakamura, T., Ohshima, T., Tanaka, S., and Matsuo, H. (1988) Yeast KEX2 gene encodes an endopeptidase homologous to subtilisin-like serine proteases. Biochem Biophys Res Commun 156: 246-254.</Citation></Reference><Reference><Citation>Mizuno, K., Nakamura, T., Ohshima, T., Tanaka, S., and Matsuo, H. (1989) Characterization of KEX2-encoded endopeptidase from yeast Saccharomyces, cerevisiae. Biochem Biophys Res Commun 159: 305-311.</Citation></Reference><Reference><Citation>Mullins, E.D., Chen, X., Romaine, P., Raina, R., Geiser, D.M., and Kang, S. (2001) Agrobacterium-mediated transformation of Fusarium oxysporum: an efficient tool for insertional mutagenesis and gene transfer. Phytopathology 91: 173-180.</Citation></Reference><Reference><Citation>Murashige, T., and Skoog, F. (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15: 473-497.</Citation></Reference><Reference><Citation>Paschlewski and Paschlewskia. 1974. Studies on symbiotic properties of mycorrhizal fungi of pine (Pinus sylvestris L.) with the aid of the method of mycorrhizal synthesis in pure culture on agar. (Translated into English by Royal Dzieciolowski). Warsaw, Pol. For. Res. Inst.</Citation></Reference><Reference><Citation>Pellegrin, C., Morin, E., Martin, F.M., and Veneault-Fourrey, C. (2015) Comparative analysis of secretomes from ectomycorrhizal fungi with an emphasis on small-secreted proteins. Front Microbiol 6: 1278. https://doi.org/10.3389/fmicb.2015.01278.</Citation></Reference><Reference><Citation>Petersen, T.N., Brunak, S., von Heijne, G., and Nielsen, H. (2011). SignalP4.0: discriminating signal peptides from transmembrane regions. Nature Methods 8: 785-786.</Citation></Reference><Reference><Citation>Peterson, A.R.L., and Farquhar, M.L. (1994) Mycorrhizas: integrated development between roots and fungi. Mycologia 86: 311-326.</Citation></Reference><Reference><Citation>Pfaffl, M.W. (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: 45e-45e.</Citation></Reference><Reference><Citation>Plett, J.M., and Martin, F. (2015) Reconsidering mutualistic plant-fungal interactions through the lens of effector biology. Curr Opin Plant Biol 26: 45-50.</Citation></Reference><Reference><Citation>Plett, J.M., Kemppainen, M., Kale, S.D., Kohler, A., Legué, V., Brun, A., et al. (2011) A secreted effector protein of Laccaria bicolor is required for symbiosis development. Curr Biol 21: 1197-1203.</Citation></Reference><Reference><Citation>Plett, J.M., Daguerre, Y., Wittulsky, S., Vayssieres, A., Deveau, A., Melton, S.J., et al. (2014) Effector MiSSP7 of the mutualistic fungus Laccaria bicolor stabilizes the Populus JAZ6 protein and represses jasmonic acid (JA) responsive genes. Proc Natl Acad Sci 111: 8299-8304.</Citation></Reference><Reference><Citation>Plett, J.M., Tisserant, E., Brun, A., Morin, E., Grigoriev, I.V., Kuo, A., et al. (2015) The Mutualist Laccaria bicolor expresses a core gene regulon during the colonization of diverse host plants and a variable regulon to counteract host-specific defenses. Mol Plant Microbe Interact 28: 261-273.</Citation></Reference><Reference><Citation>Ruxton, G.D., and Beauchamp, G. (2008) Time for some a priori thinking about post hoc testing. Behav Ecol 19: 690-693. https://doi.org/10.1093/beheco/arn020.</Citation></Reference><Reference><Citation>Shen, A., Lupardus, P.J., Morell, M., Ponder, E.L., Sadaghiani, A.M., Garcia, K.C., and Bogyo, M. (2009) Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag. PLoS One 4: e8119. https://doi.org/10.1371/journal.pone.0008119.</Citation></Reference><Reference><Citation>Teertstra, W.R., Deelstra, H.J., Vranes, M., Bohlmann, R., Kahmann, R., Kamper, J., and Wosten, H.A.B. (2006) Repellents have functionally replaced hydrophobins in mediating attachment to a hydrophobic surface and in formation of hydrophobic aerial hyphae in Ustilago maydis. Microbiology 152: 3607-3612.</Citation></Reference><Reference><Citation>Tian, X., He, G.J., Hu, P., Chen, L., Tao, C., Cui, Y.L., et al. (2018) Cryptococcus neoformans sexual reproduction is controlled by a quorum sensing peptide. Nat Microbiol 3: 698-707. https://doi.org/10.1038/s41564-018-0160-4.</Citation></Reference><Reference><Citation>Umemura, M., Nagano, N., Koike, H., Kawano, J., Ishii, T., Miyamura, Y., et al. (2014) Characterization of the biosynthetic gene cluster for the ribosomally synthesized cyclic peptide ustiloxin B in Aspergillus flavus. Fungal Genet Biol 68: 23-30.</Citation></Reference><Reference><Citation>Uroz, S., Buée, M., Murat, C., Frey-Klett, P., and Martin, F. (2010) Pyrosequencing reveals a contrasted bacterial diversity between oak rhizosphere and surrounding soil. Environ Microbiol Rep 2: 281-288.</Citation></Reference><Reference><Citation>Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., and Speleman, rank. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: 31-34.</Citation></Reference><Reference><Citation>Vayssières, A., Pěnčík, A., Felten, J., Kohler, A., Ljung, K., Martin, F., and Legué, V. (2015) Development of the poplar-Laccaria bicolor Ectomycorrhiza modifies root auxin metabolism, signaling, and response. Plant Physiol 169: 890-902.</Citation></Reference><Reference><Citation>Verstrepen, K., Jansen, A., Lewitter, F., and Fink, G. (2005) Intragenic tandem repeats generate functional variability. Nature Genet 37: 986-990.</Citation></Reference><Reference><Citation>Wawra, S., Fesel, P., Widmer, H., Timm, M., Seibel, J., Leson, L., et al. (2016) The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants. Nat Commun 7: 13188.</Citation></Reference><Reference><Citation>Welch, B. L. (1947). “The generalization of “Student's” problem when several different population variances are involved”. Biometrika 34: 28-35. doi:10.1093/biomet/34.1-2.28. MR 0019277.</Citation></Reference><Reference><Citation>Wödsten, H.A.B., Bohlmann, R., Eckerskorn, C., Lottspeich, F., Bolker, M., and Kahmann, R. (1996) A novel class of small amphipathic peptides affect aerial hyphal growth and surface hydrophobicity in Ustilago maydis. EMBO J 15: 4274-4281.</Citation></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Argentine</li><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li><li>Midi-Pyrénées</li><li>Occitanie (région administrative)</li></region><settlement><li>Castanet Tolosan</li><li>Champenoux</li><li>Metz</li><li>Nancy</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><country name="France"><region name="Grand Est"><name sortKey="Pellegrin, Clement" sort="Pellegrin, Clement" uniqKey="Pellegrin C" first="Clément" last="Pellegrin">Clément Pellegrin</name></region><name sortKey="Daguerre, Yohann" sort="Daguerre, Yohann" uniqKey="Daguerre Y" first="Yohann" last="Daguerre">Yohann Daguerre</name><name sortKey="Daguerre, Yohann" sort="Daguerre, Yohann" uniqKey="Daguerre Y" first="Yohann" last="Daguerre">Yohann Daguerre</name><name sortKey="Frey, Nicolas Frei Dit" sort="Frey, Nicolas Frei Dit" uniqKey="Frey N" first="Nicolas Frei Dit" last="Frey">Nicolas Frei Dit Frey</name><name sortKey="Guinet, Frederic" sort="Guinet, Frederic" uniqKey="Guinet F" first="Frédéric" last="Guinet">Frédéric Guinet</name><name sortKey="Guinet, Frederic" sort="Guinet, Frederic" uniqKey="Guinet F" first="Frédéric" last="Guinet">Frédéric Guinet</name><name sortKey="Hecker, Arnaud" sort="Hecker, Arnaud" uniqKey="Hecker A" first="Arnaud" last="Hecker">Arnaud Hecker</name><name sortKey="Hecker, Arnaud" sort="Hecker, Arnaud" uniqKey="Hecker A" first="Arnaud" last="Hecker">Arnaud Hecker</name><name sortKey="Martin, Francis M" sort="Martin, Francis M" uniqKey="Martin F" first="Francis M" last="Martin">Francis M. Martin</name><name sortKey="Martin, Francis M" sort="Martin, Francis M" uniqKey="Martin F" first="Francis M" last="Martin">Francis M. Martin</name><name sortKey="Pellegrin, Clement" sort="Pellegrin, Clement" uniqKey="Pellegrin C" first="Clément" last="Pellegrin">Clément Pellegrin</name><name sortKey="Puech Pages, Virginie" sort="Puech Pages, Virginie" uniqKey="Puech Pages V" first="Virginie" last="Puech-Pagès">Virginie Puech-Pagès</name><name sortKey="Ruytinx, Joske" sort="Ruytinx, Joske" uniqKey="Ruytinx J" first="Joske" last="Ruytinx">Joske Ruytinx</name><name sortKey="Ruytinx, Joske" sort="Ruytinx, Joske" uniqKey="Ruytinx J" first="Joske" last="Ruytinx">Joske Ruytinx</name><name sortKey="Veneault Fourrey, Claire" sort="Veneault Fourrey, Claire" uniqKey="Veneault Fourrey C" first="Claire" last="Veneault-Fourrey">Claire Veneault-Fourrey</name><name sortKey="Veneault Fourrey, Claire" sort="Veneault Fourrey, Claire" uniqKey="Veneault Fourrey C" first="Claire" last="Veneault-Fourrey">Claire Veneault-Fourrey</name></country><country name="Argentine"><noRegion><name sortKey="Kemppainen, Minna" sort="Kemppainen, Minna" uniqKey="Kemppainen M" first="Minna" last="Kemppainen">Minna Kemppainen</name></noRegion><name sortKey="Pardo, Alejandro G" sort="Pardo, Alejandro G" uniqKey="Pardo A" first="Alejandro G" last="Pardo">Alejandro G. 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